polyclonal antibodies against cd206  (Proteintech)

Journal: Experimental and Therapeutic Medicine

Article Title: Protective effects of budesonide on LPS-induced podocyte injury by modulating macrophage M1/M2 polarization: Evidence from in vitro and in silico studies

doi: 10.3892/etm.2022.11526

Figure Lengend Snippet: Effects of Bud on CD206, Arg-1, TNF-α and iNOS protein expression in LPS-induced RAW264.7 cells. (A) CD206, Arg-1 and TNF-α protein expression. (B) iNOS protein expression. The results of CD206, Arg-1, TNF-α and iNOS are represented in (C), (D), (E) and (F), respectively. All results were expressed as a ratio with respect to the control and represented as the mean ± SD (n=3). # P<0.05, LPS vs. normal. * P<0.05, Cur and Bud vs. LPS. ^P<0.05, Bud vs. normal. Bud, budesonide; CD206, mannose receptor; Arg-1, arginase-1; iNOS, inducible nitric oxide synthase; LPS, lipopolysaccharide; Bud5, Bud 5 µM; Bud10, Bud 10 µM; Bud20, Bud 20 µM; Cur, curcumin.

Article Snippet: After immersing the membranes into 5% nonfat dried milk (diluted in 0.1% (v/v) Tween-20 PBS) for 2 h at room temperature to block nonspecific binding, the membranes were incubated overnight with a primary antibody against iNOS at a 1:2,000 dilution (cat. no. 18985-1-AP; ProteinTech Group, Inc.), a primary antibody against TNF-α (cat. no. bs-2081R; Bioss) at a 1:1,000 dilution, a primary antibody against CD206 (cat. no. bs-21473R; Bioss) at a 1:1,000 dilution and a primary antibody against Arg-1 (cat. no. 16001-1-AP; ProteinTech Group, Inc.) at a 1:5,000 dilution at 4˚C.

Techniques: Expressing

Journal: Materials Today Bio

Article Title: Isoliquiritigenin micellar microneedle for pH monitoring and diabetic wound healing

doi: 10.1016/j.mtbio.2025.102356

Figure Lengend Snippet: Anti-inflammatory mechanism and regulation of polarization of macrophages by LSI-GCA. (a) Immunofluorescence staining for M1 marker CD86 (green: CD86, blue: DAPI). Scale bar: 100 μm. (b) Quantitative statistics of CD86. (c) Immunofluorescence staining of M2 marker CD206 (green: CD206, blue: DAPI). Scale bar: 100 μm. (d) Quantitative statistics of CD206. (e) NRF2 nuclear translocation assay (red: NRF2, blue: DAPI). Scale bar: 100 μm. (f) Quantitative statistics of NRF2 nuclear translocation assay. Data are presented as mean ± standard deviation (n = 3). (Statistical differences: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, NS, no statistical significance). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Human umbilical vein endothelial cells (HUVEC), mouse fibroblasts (3T3), and mouse macrophage RAW264.7 cells were supplied by Wuhan Pulisai Biotechnology Co., Ltd. Polyclonal antibodies against CD206 and CD86, plus CoraLite488-conjugated goat anti-rabbit IgG(H + L), were bought from Proteintech.

Techniques: Immunofluorescence, Staining, Marker, Nuclear Translocation Assay, Standard Deviation

Journal: Materials Today Bio

Article Title: Isoliquiritigenin micellar microneedle for pH monitoring and diabetic wound healing

doi: 10.1016/j.mtbio.2025.102356

Figure Lengend Snippet: LSI-GCA enhances wound angiogenesis and downregulates inflammatory factor expression. (a) TNF-α expression in skin wound tissues. Scale bar: 100 μm. (b) IL-6 expression in skin wound tissues. Scale bar: 100 μm. (c) Immunofluorescence staining of CD31 and α-SMA. Scale bar: 100 μm. (d) Immunofluorescence staining of CD206 and F4/80. Scale bar: 100 μm. (e) Quantitative analysis of TNF-α expression. (f) Quantitative analysis of IL-6 expression. (g) Quantitative analysis of CD31 expression. (h) Quantitative analysis of CD206 expression. Data are expressed as mean ± standard deviation (n = 3). (Statistical significance: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001; NS, not significant).

Article Snippet: Human umbilical vein endothelial cells (HUVEC), mouse fibroblasts (3T3), and mouse macrophage RAW264.7 cells were supplied by Wuhan Pulisai Biotechnology Co., Ltd. Polyclonal antibodies against CD206 and CD86, plus CoraLite488-conjugated goat anti-rabbit IgG(H + L), were bought from Proteintech.

Techniques: Expressing, Immunofluorescence, Staining, Standard Deviation

Journal: Frontiers in Immunology

Article Title: The interplay of inflammation and placenta in maternal diabetes: insights into Hofbauer cell expression patterns

doi: 10.3389/fimmu.2024.1386528

Figure Lengend Snippet: Hofbauer cells in the placenta. The routine staining shows typical morphology and localization of HBCs. Their presence was confirmed using CD206. All microphotographs are magnified 200x, details are 400x. HBCs are marked by arrowheads.

Article Snippet: The following primary antibodies were used: rabbit polyclonal antibodies against CD206 (Abcam; ab64693) at a dilution of 1:1000, IL-10 (Abcam; ab34843) at a dilution of 1:400, IL-1β (Novus Biologicals, NBP1-19775) at a dilution of 1:100, CYP2C8 (ProteinTech, 16546-1-AP) at a dilution of 1: 50 and CYP2J2 (Abcam, ab151996) at 1:100 dilution and mouse monoclonal antibody against CD86 (Abcam; ab220188) at 1:50 dilution, CYP2C9 (Novus Biologicals, NBP2-01381) at 1:400 dilution and sEH (Santa Cruz, sc166916) at 1:200 dilution.

Techniques: Staining